Framework mutations study published in mAbs

Very fruitful collaboration with the lab of Sophia Karagiannis at King’s College London! We present here a computational–experimental workflow to rationally design framework (FW) mutations in antibodies. Using trastuzumab as an test case, we identified and validated mutations outside the complementarity-determining regions (CDRs) that improved stability while preserving antigen binding. In one case the mutation decoupled antigen binding from Fc‑mediated effector functions, offering novel avenues for fine‑tuning antibody behaviour.

Read the paper on mAbs.